Active learning-guided optimization of large gene insertion effectors in mammalian cells​

Insertion of large genes directly into the human genome holds significant therapeutic potential. This presentation shows that an active learning-guided framework can probe large protein activity landscapes to find more functional variants in relevant mammalian cell contexts, a task traditionally performed in prokaryotes. ElevateBio is now poised to use active learning to complement traditional approaches to engineer more potent large serine recombinases and R2 retrotransposons for potent and specific gene insertion.