Next‑generation enzymes for targeted gene insertion

This presentation showcases ElevateBio's comprehensive targeted large gene insertion technology, spanning novel large serine recombinases (LSRs) and R2 retrotransposons. From a diverse catalog of over 10 billion proteins, we identified 100+ active LSRs and active R2 retrotransposons. We achieved robust, specific insertion in primary human T cells, demonstrating a promising targeted insertion platform for in vivo or ex vivo therapeutic application. We are now leveraging generative AI to make large gene insertion fully programmable by designing purpose-built LSR proteins for specific genomic targets.